The effect of resistance training on gut microbiota composition has not been explored, despite the evidence about endurance exercise. The aim of this study was to compare the effect of resistance and endurance training on gut microbiota composition in mice.
Methods: Cecal samples were collected from 26 C57BL/6N mice, divided into three groups: sedentary (CTL), endurance training on a treadmill (END), and resistance training on a vertical ladder (RES). After 2 weeks of adaption, mice were trained for 4 weeks, 5 days/week. Maximal endurance and resistance capacity test were performed before and after training. Genomic DNA was extracted and 16S Ribosomal RNA sequenced for metagenomics analysis. The percentages for each phylum, class, order, family, or genus/species were obtained using an open-source bioinformatics pipeline.
Results: END showed higher diversity and evenness. Significant differences among groups in microbiota composition were only observed at genera and species level. END showed a significantly higher relative abundance of Desulfovibrio and Desulfovibrio sp., while Clostridium and C. cocleatum where higher for RES.
Trained mice showed significantly lower relative abundance of Ruminococcus gnavus and higher of the genus Parabacteroides compared to CTL. We explored the relationship between relative taxa abundance and maximal endurance and resistance capacities after the training period.
Lachnospiraceae and Lactobacillaceae families were negatively associated with endurance performance, while several taxa, including Prevotellaceae family, Prevotella genus, and Akkermansia muciniphila, were positively correlated. About resistance performance, Desulfovibrio sp. was negatively correlated, while Alistipes showed a positive correlation.
Conclusion: Resistance and endurance training differentially modify gut microbiota composition in mice, under a high-controlled environment. Interestingly, taxa associated with anti- and proinflammatory responses presented the same pattern after both models of exercise. Furthermore, the abundance of several taxa was differently related to maximal endurance or resistance performance, most of them did not respond to training.
Link: https://www.frontiersin.org/articles/10.3389/fphys.2021.748854/full
Authors: Javier Fernández1,2,3†, Manuel Fernández-Sanjurjo2,4†, Eduardo Iglesias-Gutiérrez2,4, Pablo Martínez-Camblor5, Claudio J. Villar1,2,3, Cristina Tomás-Zapico2,4*, Benjamin Fernández-García2,6‡ and Felipe Lombó1,2,3‡
Source:
- 1Department of Functional Biology, Microbiology, University of Oviedo, Oviedo, Spain
- 2Health Research Institute of the Principality of Asturias (ISPA), Oviedo, Spain
- 3Instituto Universitario de Oncología del Principado de Asturias (IUOPA), University of Oviedo, Oviedo, Spain
- 4Department of Functional Biology, Physiology, University of Oviedo, Oviedo, Spain
- 5Department of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Hanover, NH, United States
- 6Department of Morphology and Cell Biology, Anatomy, University of Oviedo, Oviedo, Spain
Data Availability Statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found at: https://www.ncbi.nlm.nih.gov/, PRJNA558220.
Ethics Statement
The animal study was reviewed and approved by the Research Ethics Committee of the University of Oviedo, Spain (PROAE 10/2016).
Author Contributions
JF and MF-S performed the experiments, analyzed the data, and wrote the manuscript. CT-Z and PM-C analyzed the data, prepared the figures, and wrote the manuscript. CJV analyzed the data. EI-G, FL, and BF-G designed and supervised the study and wrote the manuscript. All authors have read and approved the final version of the manuscript and agree with the order of the presentation of the authors.
Funding
This work was supported by Ministerio de Economía y Competitividad under Grant DEP2015-69980-P to BF-G and by Programa de Ayudas a Grupos de Investigación del Principado de Asturias to FL (FC-GRUPIN-IDI/2018/000120).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
The authors acknowledge the technical support provided by Servicios Científico-Técnicos de la Universidad de Oviedo and the Biostatistics and Epidemiology Unit from ISPA.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphys.2021.748854/full#supplementary-material
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Keywords: resistance exercise, endurance exercise, murine models, metagenomics, physical performance
Citation: Fernández J, Fernández-Sanjurjo M, Iglesias-Gutiérrez E, Martínez-Camblor P, Villar CJ, Tomás-Zapico C, Fernández-García B and Lombó F (2021) Resistance and Endurance Exercise Training Induce Differential Changes in Gut Microbiota Composition in Murine Models. Front. Physiol. 12:748854. doi: 10.3389/fphys.2021.748854
Received: 09 August 2021; Accepted: 01 December 2021;
Published: 24 December 2021.
Edited by:
Silvia Turroni, University of Bologna, Italy
Reviewed by:
Jing Li, Shandong University, China
Monica Barone, University of Bologna, Italy
Copyright © 2021 Fernández, Fernández-Sanjurjo, Iglesias-Gutiérrez, Martínez-Camblor, Villar, Tomás-Zapico, Fernández-García and Lombó. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Cristina Tomás-Zapico, tomascristina@uniovi.es
†These authors have contributed equally to this work and share first authorship
‡These authors have contributed equally to this work and share senior authorship
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Nutrigenomics Institute is not responsible for the comments and opinions included in this article
